Roya Hedayati Kashka; Saeed Zavareh; Taghi Lashkabolouki
Volume 23, Issue 4 , September and October 2016, , Pages 590-599
Abstract
Background Oxidative stress is unavoidable during in vitro culture. The present study aimed to investigate the effect of ubiquinone as a potent antioxidant on lipid peroxidation and development rate of mice in vitro cultured preantral follicles.Materials & Methods Preantral follicles were isolated ...
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Background Oxidative stress is unavoidable during in vitro culture. The present study aimed to investigate the effect of ubiquinone as a potent antioxidant on lipid peroxidation and development rate of mice in vitro cultured preantral follicles.Materials & Methods Preantral follicles were isolated mechanically from 14- to 16-day-old mice (n=123) and divided into control (n=78) and ubiquinone treated groups (n=45). Preantral follicles were cultured in the presence or absence (control) of 50 µM ubiquinone. Ovulation was induced at 12th day. The rates of growth, survival, antrum formation, ovulation, and MII oocytes were evaluated. Separately, malondialdehyde (MDA) as a biomarker of lipid peroxidation was assessed at different time points of 24, 48, 72, and 96 h. Statistical analysis was performed by Independent t test through using SPSS.Results The growth and survival rate of ubiquinone treated preantral follicles was significantly higher than those of the control group. The rates of antrum formation, ovulation, and MII oocytes in the presence of ubiquinone were significantly higher than those of the control group. Whereas, MDA levels of ubiquinone treated preantral follicles was significantly lower compared with that of the control group.Conclusion Supplemented culture medium with ubiquinone improves the development of preantral follicles by reducing the lipid peroxidation.
Nasibeh Akbari; Mahmoud Allahdadi Dalmani; Taghi Lashkar Blouki; Mehdi Godarzvand
Volume 23, Issue 1 , May and June 2016, , Pages 66-74
Abstract
Purpose: Epilepsy, as a plastic change lead to hyperexcitability and structural change. Kindling is the process of repetitive subconvulsive electrical or chemical stimulation induced synaptic and circuit alterations. Lateral hypothalamus (LH) contains the main constellation of orexinergic neurons involved ...
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Purpose: Epilepsy, as a plastic change lead to hyperexcitability and structural change. Kindling is the process of repetitive subconvulsive electrical or chemical stimulation induced synaptic and circuit alterations. Lateral hypothalamus (LH) contains the main constellation of orexinergic neurons involved in sleep and waking and even excitability with high numbers of receptors in hippocampus. Thus, we investigate the effect of LH inactivation on kindling development and kindling induced hippocampal neuronal population. Method: Pentylenetetrazol (PTZ; 45 mg/kg) was used to induce chemical kindling every 48 h up to 13 injections, intraperitoneally. Three consecutive 4 or 5 seizure stages were criteria for kindling. Lidocaine (2%) was injected stereotaxically into right LH, 0.5 h prior to PTZ administration. Nissl staining was used to demonstrate neuronal population survival in CA3 and hilar regions of hippocampus. Results: LH inactivation prevented PTZ kindling development. Although kindling increased neuronal dispersion in CA3 and hilar regions, LH inactivation was unable to reduce the dispersion. Moreover, kindling did not affect neuronal survival in CA3 and hilus, qualitatively. Conclusion: It is concluded that kindling induced structural changes is to some extent independent of kindling development and it is not prevented by kindling inhibition through LH inactivation.